An Agarose-Gel Based Method for Transporting Cell Lines
Lingzhi Yang 1, 2, Chufang Li1, Ling Chen1, 2, Zhiyuan Li*, 1, 2
Identifiers and Pagination:Year: 2009
First Page: 50
Last Page: 53
Publisher Id: CCGTM-3-50
Article History:Received Date: 5/5/2009
Revision Received Date: 21/6/2009
Acceptance Date: 19/9/2009
Electronic publication date: 16/12/2009
Collection year: 2009
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.
Cryopreserved cells stored in dry ice or liquid nitrogen is the classical method for transporting cells between research laboratories in different cities around the world in order to maintain cell viability. An alternative method is to ship the live cells in flasks filled with cell culture medium. Both methods have limitations of either a requirement on special shipping container or short times for the cells to survive on the shipping process. We have recently developed an agarose gel based method for directly transporting the live adherent cells in cell culture plates or dishes in ambient temperature. This convenient method simplifies the transportation of live cells in long distance that can maintain cells in good viability for several days.