In Vivo Fluorescent Labeling of Tumor Cells with the HaloTag® Technology
Jen-Chieh Tseng*, 1, Hélène A Benink3, Mark G McDougall4, Isabel Chico-Calero2, Andrew L Kung1 , 2
Identifiers and Pagination:Year: 2012
Issue: Suppl 1
First Page: 48
Last Page: 54
Publisher Id: CCGTM-6-48
Article History:Received Date: 30/3/2012
Revision Received Date: 17/5/2012
Acceptance Date: 18/5/2012
Electronic publication date: 20/9/2012
Collection year: 2012
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Many fluorescent sensors are currently available for in vitro bio-physiological microscopic imaging. The ability to label cells in living animals with these fluorescent sensors would help translate some of these assays into in vivo applications. To achieve this goal, the first step is to establish a method for selectively labeling target cells with exogenous fluorophores. Here we tested whether the HaloTag® protein tagging system provides specific labeling of xenograft tumors in living animals. After systemic delivery of fluorophore-conjugated ligands, we performed whole animal planar fluorescent imaging to determine uptake in tag-expressing HCT116 xenografts. Our results demonstrate that HaloTag ligands containing red or near-infrared fluorophores have enhanced tumor uptake and are suitable for non-invasive in vivo imaging. Our proof-of-concept results establish feasibility for using HaloTag technology for bio-physiological imaging in living animals.